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Promega rt buffer promega
Rt Buffer Promega, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt buffer promega - by Bioz Stars, 2026-10
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Reverse Transcription:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.

cDNA Synthesis:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.

Purification:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.

FACS:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.

Polymerase Chain Reaction:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.

Sequencing:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.

Random Hexamer:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.

Sterility:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.

Virus:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.

Incubation:

Article Title: Chaihu-shugan san inhibits inflammatory response to improve insulin signaling in liver and prefrontal cortex of CUMS rats with glucose intolerance.
Article Snippet: Depression is a mental illness comorbid risk factor for glucose intolerance worldwide.. Chaihu-shugan san, a ‘ShuGan’ formula in traditional Chinese medicine, is clinically used in the treatment of depression.. The aim of this study was to investigate whether Chaihu-shugan san improved glucose tolerance with its antidepressant activity in rat model of depression and explore the mechanisms underlying its action on liver-brain inflammation axis.

Article Title: Artificial vascular grafts, their construction and use
Article Snippet: A mixture of 0.4 mM dNTPs, 5 units AMV-reverse transcriptase (RT) (Promega), 5 mM DTT, 32 units RNAse-out (Gibco BRL) and RT buffer (Promega) was added to the RNA.

Article Title: N-myc downstream-regulated gene 2 (NDRG2) suppresses the epithelial-mesenchymal transition (EMT) in breast cancer cells via STAT3/Snail signaling.
Article Snippet: Although NDRG2 has recently been found to be a candidate tumor suppressor, its precise role in the epithelial–mesenchymal transition (EMT) is not well understood.. In the present study, we demonstrated that NDRG2 overexpression in MDA-MB-231 cells down-regulated the expression of Snail, a transcriptional repressor of E-cadherin and a key regulator of EMT, as well as the phosphorylation of signal transducer and activator of transcription 3 (STAT3), an oncogenic transcription factor that is activated in many human malignancies including breast cancer.. In addition, we confirmed that the expression of Snail and phospho-STAT3 was recovered when NDRG2 was knocked down by siRNA in MCF7 cells in which NDRG2 is endogenously expressed.

Article Title: Expression of different survivin variants in gastric carcinomas: first clues to a role of survivin-2B in tumour progression
Article Snippet: Total RNA (2 μg) was reversely transcribed in a final 30 μl reaction volume using 5 U AMV reverse transcriptase (Promega, Heidelberg, Germany), 1×RT buffer (Promega), 20 U of RNase inhibitor RNAsin (Promega), 25 μ M of each deoxynucleotide triphosphate (Qiagen, Hilden, Germany) and 10 pmol of each sequence-specific RT primer.

Article Title: Anticancer Effect of E26 Transformation-Specific Homologous Factor through the Induction of Senescence and the Inhibition of Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells.
Article Snippet: Oligo-dT, RT buffer (Promega, Madison, WI, USA), dNTPs (Bioneer, Daejeon, Republic of Korea), and M-MLV reverse transcriptase (Promega) were used for cDNA synthesis.

Article Title: The L3 of Brugia induces a Th2-polarized response following activation of an IL-4-producing CD4-CD8- alphabeta T cell population.
Article Snippet: 2 μl (1 μg) of random hexamer primers (Promega) and 1 μl of RNAsin (Promega) were added to 11 μl (~2 μg) of total RNA, and the mix was heated to 65°C for 5 min and then cooled on ice for 5 min; 5 μl of RT buffer (Promega), 2.5 μl of 10 μM dNTP mix (Perkin-Elmer), 2.5 μl of double distilled, sterile H2O and 1 μl of murine Moloney leukemia virus RT (Promega) were added and incubated at 42°C for 45 min.

Article Title: Glomerular mitochondrial changes in HIV associated renal injury.
Article Snippet: Please cite this article as: Joseph L. Bryant, Poornachander R. Guda, Girma Asemu, Rogin Subedi, Sugata Ray, Omar S. Khalid, Vivek Shukla, Dhruvil Patel, Harry Davis, Vamshi K.C.. Nimmagadda, Tapas K. Makar , Glomerular mitochondrial changes in HIV associated renal injury.. The address for the corresponding author was captured as affiliation for all authors.

Article Title: Biallelic Germline Transcription at the κ Immunoglobulin Locus
Article Snippet: Individual B220 + cells were FACS ® sorted directly into 12.5 μl RT buffer (1× RT buffer [Promega], 20 U Rnasin [Promega], 500 ng oligo dT (GIBCO BRL) and 100 ng RT primer RMB1: 5′-CACTCATTCCTGTTGAAGCTCTTG-3′) in individual PCR tubes.



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Image Search Results


Journal: iScience

Article Title: Depletion of cardiac cardiolipin synthase alters systolic and diastolic function

doi: 10.1016/j.isci.2021.103314

Figure Lengend Snippet:

Article Snippet: M-MLV RT 5x Buffer , Promega , M5313.

Techniques: Control, Recombinant, SYBR Green Assay, Fluorescence, Mutagenesis, Knockdown, Software

Journal: Cell Reports

Article Title: Peroxisome Proliferator-Activated Receptor γ2 Controls the Rate of Adipose Tissue Lipid Storage and Determines Metabolic Flexibility

doi: 10.1016/j.celrep.2018.07.063

Figure Lengend Snippet:

Article Snippet: M-MLV RT 5x buffer , Promega , M351A.

Techniques: Recombinant, Reverse Transcription, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Control, Software

Journal: Cell Metabolism

Article Title: Short-Term Fasting Reveals Amino Acid Metabolism as a Major Sex-Discriminating Factor in the Liver

doi: 10.1016/j.cmet.2018.05.021

Figure Lengend Snippet:

Article Snippet: M-MLV RT and 5X Buffer , Promega , M531A.

Techniques: Virus, Recombinant, Sample Prep, Reverse Transcription, dsDNA Assay, Coomassie Assay, Control, Transgenic Assay, Plasmid Preparation, Software